A Study of the Contribution of Changes in the Cerebral Blood Volume to the Haemodynamic Response to Anoxia in Rat Brain

1996 ◽  
Vol 9 (6) ◽  
pp. 233-240 ◽  
Author(s):  
Richard A. Jones ◽  
Olav Haraldseth ◽  
António M. Baptista ◽  
Tomm B. Müller ◽  
Audun N. Øksendal
Keyword(s):  
Rat Brain ◽  
Blood Volume ◽  
Cerebral Blood Volume ◽  
Haemodynamic Response

A Study of the contribution of changes in the cerebral blood volume to the haemodynamic response to anoxia in rat brain

1997 ◽  
Vol 10 (2) ◽  
pp. 59-66 ◽  
Author(s):  
Richard A. Jones ◽  
Olav Harakdseth ◽  
António M. Baptista ◽  
Tomm B. Müller ◽  
Audun N. Øksendal
Keyword(s):  
Rat Brain ◽  
Blood Volume ◽  
Cerebral Blood Volume ◽  
Haemodynamic Response

DETECTION OF Ca2+-DEPENDENT NEURONAL ACTIVITY SIMULTANEOUSLY WITH DYNAMIC CHANGES IN CEREBRAL BLOOD VOLUME AND TISSUE OXYGENATION FROM THE LIVE RAT BRAIN

2009 ◽  
Vol 02 (02) ◽  
pp. 189-200 ◽  
Author(s):  
CONGWU DU ◽  
ZHONGCHI LUO ◽  
MEI YU ◽  
HELENE BENVENISTE ◽  
MELISSA TULLY ◽  
...  
Keyword(s):  
Rat Brain ◽  
Blood Volume ◽  
Neuronal Activity ◽  
Cerebral Blood Volume ◽  
Tissue Oxygenation ◽  
Simultaneous Detection ◽  
Fluorescence Emission ◽  
High Temporal Resolution ◽  
Functional Changes ◽  
The Brain

We present a catheter-based optical diffusion and fluorescence (ODF) probe to study the functional changes of the brain in vivo. This ODF probe enables the simultaneous detection of the multi-wavelength absorbance and fluorescence emission from the living rat brain. Our previous studies, including a transient stroke experiment of the rat brain as well as the brain response to cocaine, have established the feasibility of simultaneously determining changes in cerebral blood volume (CBV), tissue oxygenation ( S t O 2) and intracellular calcium ([ Ca 2+]i, using the fluorescence indicator Rhod2). Here, we present our preliminary results of somatosensory response to electrical forepaw stimulation obtained from the rat cortical brain by using the ODF probe, which indicate that the probe could track brain activation by directly detecting [ Ca 2+]i along with separately distinguishing CBV and S t O 2 in real time. The changes of CBV, S t O 2 and [ Ca 2+]i are comparable with the blood-oxygen-level-dependent (BOLD) response to the stimulation obtained using functional magnetic resonance imaging (fMRI). However, the high temporal resolution of the optical methodology is advanced, thus providing a new modality for brain functional studies to understand the hemodynamic changes that underlie the neuronal activity.

scholarly journals Quantitative Assessment of the Balance between Oxygen Delivery and Consumption in the Rat Brain after Transient Ischemia with T2-BOLD Magnetic Resonance Imaging

2002 ◽  
Vol 22 (3) ◽  
pp. 262-270 ◽  
Author(s):  
Mikko I. Kettunen ◽  
Olli H. J. Gröhn ◽  
M. Johanna Silvennoinen ◽  
Markku Penttonen ◽  
Risto A. Kauppinen
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Carotid Artery ◽  
Rat Brain ◽  
Blood Volume ◽  
Common Carotid Artery ◽  
Cerebral Blood Volume ◽  
Blood Oxygenation ◽  
Resonance Imaging ◽  
Transient Ischemia

The balance between oxygen consumption and delivery in the rat brain after exposure to transient ischemia was quantitatively studied with single-spin echo T2-BOLD (blood oxygenation level–dependent) magnetic resonance imaging at 4.7 T. The rats were exposed to graded common carotid artery occlusions using a modification of the four-vessel model of Pulsinelli. T2, diffusion, and cerebral blood volume were quantified with magnetic resonance imaging, and CBF was measured with the hydrogen clearance method. A transient common carotid artery occlusion below the CBF value of approximately 20 mL·100 g−1·min−1 was needed to yield a T2 increase of 4.6 ± 1.2 milliseconds (approximately 9% of cerebral T2) and 6.8 ± 1.7 milliseconds (approximately 13% of cerebral T2) after 7 and 15 minutes of ischemia, respectively. Increases in CBF of 103 ± 75% and in cerebral blood volume of 29 ± 20% were detected in the reperfusion phase. These hemodynamic changes alone could account for only approximately one third of the T2 increase in luxury perfusion, suggesting that a substantial increase in blood oxygen saturation (resulting from reduced oxygen extraction by the brain) is needed to explain the magnetic resonance imaging observation.

scholarly journals Regional response of cerebral blood volume to graded hypoxic hypoxia in rat brain

2002 ◽  
Vol 89 (2) ◽  
pp. 287-293 ◽  
Author(s):  
C. Julien-Dolbec ◽  
I. Tropres ◽  
O. Montigon ◽  
H. Reutenauer ◽  
A. Ziegler ◽  
...  
Keyword(s):  
Rat Brain ◽  
Blood Volume ◽  
Cerebral Blood Volume ◽  
Hypoxic Hypoxia

scholarly journals Cerebral Blood Volume Alterations in the Perilesional Areas in the Rat Brain after Traumatic Brain Injury—Comparison with Behavioral Outcome

2010 ◽  
Vol 30 (7) ◽  
pp. 1318-1328 ◽  
Author(s):  
Riikka Immonen ◽  
Taneli Heikkinen ◽  
Leena Tähtivaara ◽  
Antti Nurmi ◽  
Taina-Kaisa Stenius ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Rat Brain ◽  
Blood Volume ◽  
Time Course ◽  
Cerebral Blood Volume ◽  
Motor Deficit ◽  
First Response ◽  
Initial Drop ◽  
Magnetic Resonance Imaging Mri

In the traumatic brain injury (TBI) the initial impact causes both primary injury, and launches secondary injury cascades. One consequence, and a factor that may contribute to these secondary changes and functional outcome, is altered hemodynamics. The relative cerebral blood volume (CBV) changes in rat brain after severe controlled cortical impact injury were characterized to assess their interrelations with motor function impairment. Magnetic resonance imaging (MRI) was performed 1, 2, 4 h, and 1, 2, 3, 4, 7, and 14 days after TBI to quantify CBV and water diffusion. Neuroscore test was conducted before, and 2, 7, and 14 days after the TBI. We found distinct temporal profile of CBV in the perilesional area, hippocampus, and in the primary lesion. In all regions, the first response was drop of CBV. Perifocal CBV was reduced for over 4 days thereafter gradually recovering. After the initial drop, the hippocampal CBV was increased for 2 weeks. Neuroscore demonstrated severely impaired motor functions 2 days after injury (33% decrease), which then slowly recovered in 2 weeks. This recovery parallelled the recovery of perifocal CBV. CBV MRI can detect cerebrovascular pathophysiology after TBI in the vulnerable perilesional area, which seems to potentially associate with time course of sensory-motor deficit.

scholarly journals MRI Reveals That Early Changes in Cerebral Blood Volume Precede Blood–Brain Barrier Breakdown and Overt Pathology in MS-like Lesions in Rat Brain

2005 ◽  
Vol 25 (2) ◽  
pp. 204-216 ◽  
Author(s):  
Kerry A Broom ◽  
Daniel C Anthony ◽  
Andrew M Blamire ◽  
Sara Waters ◽  
Peter Styles ◽  
...  
Keyword(s):  
Rat Brain ◽  
Blood Brain Barrier ◽  
Blood Volume ◽  
Cerebral Blood Volume ◽  
Brain Barrier ◽  
Delayed Type Hypersensitivity ◽  
Tissue Water ◽  
Edema Formation ◽  
Blood Brain ◽  
Regional Cerebral Blood Volume

Magnetic resonance imaging (MRI) is an established clinical tool for diagnosing multiple sclerosis (MS), the archetypal central nervous system neuroinflammatory disease. In this study, we have used a model of delayed-type hypersensitivity in the rat brain, which bears many of the hallmarks of an MS lesion, to investigate the development of MRI-detectable changes before the appearance of conventional indices of lesion development. In addition, we have correlated the MRI-detectable changes with the developing histopathology. Significant increases in regional cerebral blood volume (rCBV) preceded overt changes in blood–brain barrier (BBB) permeability, T2 relaxation and the diffusion properties of tissue water. Thus, changes in rCBV might be a more sensitive indicator of lesion onset than the conventional indices used clinically in MS patients, such as contrast enhancement. In addition, we show that BBB breakdown, and consequent edema formation, are more closely correlated with astrogliosis than any other histopathologic changes, while regions of T1 and T2 hypointensity appear to reflect hypercellularity.

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